Author: Ali Reza Mirzaei, Vida Shakoory-Moghadam
Publishing Date: 2022
E-ISSN: 2823-2550
Volume: 2
Issue: 2
DOI: https://doi.org/10.55705/cmbr.2022.336232.1035
ABSTRACT:
Different groups of enzymes and regulatory elements are involved in the synthesis of Ribodioside A, which is one of the most important sweetening compounds in stevia. The UGT family (UDPglycosyltransferase) is a group of regulatory genes that are very effective in converting steviol glycoside to Ribodioside A. Bioinformatics analyses on this gene family, which included the UGT74G1, UGT76G1, and UGT85C2 genes, showed that the protein encoded by these genes had a UDPGT protected protein domain. Also, the study of the secondary structure of these proteins showed that the total corrosion of these proteins is mainly from alpha-helix and random screws or loops that are connected with linear strands. Also, the study of the secondary structure of these proteins showed that the total corrosion of these proteins is mainly from alpha-helix and random screws or loops that are connected with linear strands. The results of studying the three-dimensional structure of the studied proteins confirmed the previous findings of high genomic similarity between these proteins. The results of the ProtScale program showed that the abundance of amino acids with negative hydropathicity in the sequence of these proteins is high, which is effective in creating plant resistance to drought stress. Finally, the codon preference trend of these proteins was investigated using the sequence manipulation suite database. This information can be used for other research, including the transfer of these proteins.
Key Words: UDP-glycosyltransferase (UGT), Bioinformatics analysis, Ribodioside A, Stevia rebaudiana