Author: Jia-Le Song, Ruo-Kun Yi and Yang Gao
Publishing Date: 2016
E-ISSN: 1011-601X
Volume: 29 Issue: 3
ABSTRACT:
The aim of this study was to investigate the potential anti-inflammatory effect of Conyzacanadeusis methanol extract (CME) using a cell model of RAW264.7 murine macrophage cell stimulated with lipopolysaccharide (LPS)(1μg/ml). Co-treatment with different concentrations (10, 50 and 100μg/ml) of CME was concentrationdependently reduced the LPS-induced generation of prostaglandin E2 (PGE2), nitric oxide (NO) tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, CME also reduced the mRNA expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), TNF-α, IL-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggested that CME showed an anti-inflammatory activity through reduced the mRNA expression of COX-2, iNOS, TNF-α, IL-1β and IL-6 and also decreased the productions of PGE2, NO, TNF-α, IL-1β and IL-6in LPS-stimulated RAW264.7 cells.
KEYWORDS: Conyzacanadensis, anti-inflammation, RAW264.7 cells.