Author: Amina Arif, Naeem Rashid, Farheen Aslam, Nasir Mahmood and Muhammad Akhtar
Publishing Date: 2016
E-ISSN: 1011-601X
Volume: 29 Issue: 2
ABSTRACT:
Human interferon α-2b and Escherichia coli methionine amino peptidase genes were cloned independently as well as bicistronically in expression plasmid pET-21a (+). Production of human interferon α-2b was comparable to that of E. coli methionine amino peptidase when these genes were expressed independently in E. coli BL21-CodonPlus (DE3)-RIL. However, human interferon α-2b was produced in a much less amount whereas there was no difference in the production of methionine amino peptidase when the encoding genes were expressed bicistronically. It is important to note that human interferon α-2b was the first gene in order, after the promoter and E. coli methionine amino peptidase was the next with a linker sequence of 27 nucleotides between them.
KEYWORDS: Human, interferon α-2b, Escherichia coli, methionine amino peptidase, bicistronic expression.