Author: Tauheeda Riaz, Muhammad Athar Abbasi, Aziz-ur-Rehman, Sabahat Zahra Siddiqui, Muhammad Shahid, Hina Fatima, Muhammad Ashraf, Syeda Abida Ejaz, Zahid Ghulam RasooL and Ghulam Hasan Abbasi
Publishing Date: 2017
E-ISSN: 1011-601X
Volume 30 Issue 1
ABSTRACT:
The purpose of the present investigation was to assess the enzyme inhibition, antifungal, antibacterial and hemolytic activities of various fractions of Colebrookia oppositifolia Smith. The MeOH extract of plant was dissolved in dist. water and partitioned with n-hexane, CHCl3, EtOAc and n-BuOH sequentially. Enzyme inhibition studies were done against four enzymes i.e. α-glucosidase, butyrylcholinesterase, acetyl cholinesterase and lipoxygenase. Ethyl acetate fraction possessed very good activity against α-glucosidase (IC50 57.38±1.23μg/mL). CHCl3 fraction displayed good activity against α-glucosidase and lipoxygenase while moderate activity against butyryl cholinesterase. EtOAc fraction displayed good activity against lipoxygenase. Antifungal activity was studied against four fungi i.e. Aspergillus niger, Aspergillus flavus, Ganoderma lucidum and Alternaria alternata by the disc diffusion method using fluconazole, a standard antifungal drug, as positive control. Aqueous fraction displayed good activity against G. lucidum and A. flavus. Antibacterial activity was checked against Staphylococcus aureus, Bacillus subtilis, Pasturella multocida and Escherichia coli by the disc diffusion method using streptomycin sulphate, a standard antibiotic, as positive control. Chloroform, ethyl acetate and aqueous fraction showed good activity against E. coli. Chloroform fraction showed good activity against B. subtilis. Ethyl acetate fraction showed good activity against the P. multocida. All the studied fractions showed very less toxicity i.e. < 7%.
KEYWORDS: Colebrookia oppositifolia Smith, enzyme inhibitory potential, antifungal potential, disc diffusion method, α- glucosidase, lipoxygenase, hemolytic effects.