Author: S. Namjou., E. Shokoohi and Z. Lorin
Publishing Date: 2013
E-ISSN: 0255-7576
Volume: 31 Issue: 1
ABSTRACT:
The reproduction of an Iranian population of Diolenchus desn-uctor Thome. 1945 was investigated in different media under laboratory conditions to identify the best medium and fungal host for mass production of this nematode. Four media, including three handmade PDA media (Potato Dextrose Agar). prepared from extracts of three cultivars of potato (Santana. Ramos & Maradona) and one medium of OMA (Oat Meal Apr). were used as substrates to grow fungi isolated from potato fields. including different anastomozis group of Rhizoctonia solani (AG3. AG4. AG5. AG6. AGD. AG9). Phytophthora capsici. Bonytis cinerea and Fusarium oxysporum f.sp. lycopersici. These fungal cultures were then inoculated with the nematodes and maintained at 20 and 25 °C. The results showed that the nematode fed and reproduced more on R. solani (AG3. AG4 and AGD) and F. caysporum Isp. oropersici than on other fungi. However. R. solani (AG3 and AG5) and P. capsici at 25 °C were also suitable for mass production of this nematode. The reproduction of the nematode was greater at 25 °C than at 20 °C.